Regulation of chondroitin sulfate synthesis. Effect of beta-xylosides on synthesis of chondroitin sulfate proteoglycan, chondroitin sulfate chains, and core protein.
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چکیده
منابع مشابه
Regulation of chondroitin sulfate synthesis. Effect of beta-xylosides on synthesis of chondroitin sulfate proteoglycan, chondroitin sulfate chains, and core protein.
Monolayer cultures of embryonic chick chondrocytes were incubated with 35SO42- in the presence and absence of 1.0 mM p-nitrophenyl-beta-d-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of beta-xyloside-initiated polysaccharide chains was accompanied by an apparent de...
متن کاملRegulation of Chondroitin Sulfate Synthesis EFFECT OF P-XYLOSIDES ON SYNTHESIS OF CHONDROITIN SULFATE PROTEOGLYCAN, CHONDROITIN SULFATE CHAINS, AND CORE PROTEIN*
Monolayer cultures of embryonic chick chondrocytes were incubated with %04*in the presence and absence of 1.0 mM p-nitrophenyl-/3-o-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of P-xyloside-initiated polysaccharide chains was accompanied by an apparent decrease in...
متن کاملIntracellular Synthesis of Chondroitin Sulfate
In autoradiograms of slices of costal cartilage, incubated for 4 hours in a salt solution containing S(35)-sulfate and then washed extensively and dehydrated, about 85 per cent of the radioactivity was assignable to the chondrocytes. From alkaline extracts of similarly prepared slices of cartilage, 64 to 83 per cent of the total sulfur-35 in the slices was isolated as chondroitin sulfate by chr...
متن کاملStimulation of synthesis of free chondroitin sulfate chains by beta-D-xylosides in cultured cells.
Beta-Xylosides stimulate 2- to 6-fold the synthesis of glycosaminoglycans by three types of nonconnective tissue cells (RG-C6, NB41A, and rat hepatoma cells, and normal and simian virus 40 (SV40)-transformed normal human skin fibroblasts. The effect, which is specific for the anomeric linkage and the glycone, is observed in the presence and absence of puromycin. Beta-Xylosides may substitute f...
متن کاملChondroitin sulfate
While some complementary and alternative techniques have been studied scientifically, high-quality data regarding safety, effectiveness, and mechanism of action are limited or controversial for most therapies. Whenever possible, it is recommended that practitioners be licensed by a recognized professional organization that adheres to clearly published standards. In addition, before starting a n...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)39958-1